Product Name	BNIP3
Chinese Name	促凋亡调节蛋白BNIP3抗体
Alias	BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3_HUMAN; NIP 3; NIP3.
literatures	Specific References  (6)     |     SL4239R has been referenced in 6 publications. [IF=5.396] Kaidi Ma. et al. Lactobacillus rhamnosus GG ameliorates deoxynivalenol-induced kidney oxidative damage and mitochondrial injury in weaned piglets. Food Funct. 2022 Mar;:  WB ;  Piglets.   PubMed:35285834 [IF=5.168] Du et al. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling. (2017) Oncotarget. 8:61510-61527  IF ;  Human.   PubMed:28977881 [IF=4.932] Qifeng Deng. et al. Cordycepin enhances anti-tumor immunity in colon cancer by inhibiting phagocytosis immune checkpoint CD47 expression. Int Immunopharmacol. 2022 Jun;107:108695  WB,IHC ;  Mouse.   PubMed:35305385 [IF=4.109] He Bai. et al. Inhibition of the BNIP3/NIX-dependent mitophagy aggravates copper-induced mitochondrial dysfunction in duck renal tubular epithelial cells. ENVIRON TOXICOL. 2022 Nov;:  WB ;  Duck.   PubMed:36378575 [IF=2.1] Ruiqiao Guan. et al. Electroacupuncture at GV20‑GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis. Mol Med Rep. 2021 Jul;24(1):1-15  WB ;  Rat.   PubMed:33955500 [IF=1.49] Qian Zhang. et al. Expression of HIF1α, BNIP3, and beclin-1 in the brain of newborn and adult yaks (Bos grunniens). FOLIA HISTOCHEM CYTO. 2023 Mar;61(1):26-33  IHC ;  Bovine.   PubMed:36987743
Research Area	Tumour  Cardiovascular  immunology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BNIP3: 101-194/194
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Function: Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. Subunit: Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains). Subcellular Location: Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Similarity: Belongs to the NIP3 family. SWISS: Q12983 Gene ID: 664 Database links: Entrez Gene: 664 Human Entrez Gene: 12176 Mouse Entrez Gene: 84480 Rat Omim: 603293 Human SwissProt: Q12983 Human SwissProt: O55003 Mouse Unigene: 144873 Human Unigene: 378890 Mouse Unigene: 2060 Rat BNIP3蛋白在缺氧条件下可诱导细胞发生Apoptosis。BNIP3促ApoptosisMitochondrion途径根据有无caspase的参与而具有不同的作用机制，也是研究的热点。BNIP3还可诱导Autophagy。BNIP3全称为Bcl2/腺病毒E1B19kD相互作用蛋白3。(Bcl2/adenovirusE1B19kDainteractingprotein3，BNIP3)，是新近发现的BH3only亚家族成员之一，也是低氧敏感的凋亡基因。在化学性低氧诱导Apoptosis的过程中，ATP是BNIP3导致Apoptosis的关键性因素之一。单独ATP下降不诱导Apoptosis，但ATP下降到一定程度能促进BNIP3向Mitochondrion转位，从而诱导了BNIP3相关的凋亡。这可能是低氧诱导BNIP3表达导致Apoptosis的重要机制之一。
Product Picture	Blank control:A431. Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (SL4239R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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