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Product Name Squalene Epoxidase Chinese Name 鲨烯环氧酶抗体 Alias ERG1; SE antibody SQLE; Squalene monooxygenase; ERG1_HUMAN. Research Area Tumour Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 64kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Squalene Epoxidase: 101-200/574 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This protein catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Function:
Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Subunit:
May form a complex with squalene synthase.
Subcellular Location:
Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Similarity:
Belongs to the squalene monooxygenase family.
SWISS:
Q14534
Gene ID:
6713
Database links:Entrez Gene: 6713 Human
Entrez Gene: 20775 Mouse
Omim: 602019 Human
SwissProt: Q14534 Human
SwissProt: P52019 Mouse
Unigene: 71465 Human
Unigene: 296169 Mouse
Unigene: 33239 Rat
Product Picture Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Squalene Epoxidase (SL4218R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Squalene Epoxidase Polyclonal Antibody, Unconjugated(SL4218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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