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Product Name Cullin 1 Chinese Name Cullin1抗体 Alias Cullin-1; CUL 1; CUL1; Cullin1; MGC149834; MGC149835. Research Area Tumour immunology Signal transduction Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Pig, Cow, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 85kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cullin 1: 701-776/776 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast.
Tissue Specificity:
Expressed in lung fibroblasts.
Post-translational modifications:
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
Similarity:
Belongs to the cullin family.
SWISS:
Q13616
Gene ID:
8454
Database links:Entrez Gene: 8454 Human
Entrez Gene: 26965 Mouse
Omim: 603134 Human
SwissProt: Q13616 Human
SwissProt: Q9WTX6 Mouse
Unigene: 146806 Human
Unigene: 87611 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cullin 1) Polyclonal Antibody, Unconjugated (SL4208R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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