Product Name	CHMP1A
Chinese Name	染色质修饰蛋白1A抗体
Alias	Charged multivesicular body protein 1a; Chromatin modifying protein 1a; hVps46 1; Metalloprotease 1; PCOLN3; Procollagen (type III) N endopeptidase; PRSM1; Vacuolar protein sorting 46 1; Vps46 1; CHM1A_HUMAN.
Research Area	Tumour  Cell biology  immunology  Signal transduction  Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CHMP1A: 51-150/196
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein PCGF4/BMI1 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins. Function: Probable peripherally associated component of theendosomal sorting required for transport complex III (ESCRT-III)which is involved in multivesicular bodies (MVBs) formation andsorting of endosomal cargo proteins into MVBs. MVBs containintraluminal vesicles (ILVs) that are generated by invagination andscission from the limiting membrane of the endosome and mostly aredelivered to lysosomes enabling degradation of membrane proteins,such as stimulated growth factor receptors, lysosomal enzymes andlipids. The MVB pathway appears to require the sequential functionof ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostlydissociate from the invaginating membrane before the ILV isreleased. The ESCRT machinery also functions in topologicallyequivalent membrane fission events, such as the terminal stages ofcytokinesis and the budding of enveloped viruses (HIV-1 and otherlentiviruses). ESCRT-III proteins are believed to mediate thenecessary vesicle extrusion and/or membrane fission activities,possibly in conjunction with the AAA ATPase VPS4. Involved incytokinesis. Involved in recruiting VPS4A and/or VPS4B to themidbody of dividing cells. May also be involved in chromosomecondensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4to regions of condensed chromatin. May play a role in stable cellcycle progression and in PcG gene silencing. Subunit: Probable peripherally associated component of theendosomal sorting required for transport complex III (ESCRT-III).ESCRT-III components are thought to multimerize to form a flatlattice on the perimeter membrane of the endosome. Several assemblyforms of ESCRT-III may exist that interact and act sequentally.Self-associates. Interacts with CHMP1B. Interacts with VPS4A.Interacts with VPS4B. Interacts with PHF1. Interacts with IST1. Subcellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus matrix. Tissue Specificity: Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG63. Similarity: Belongs to the SNF7 family. SWISS: Q9HD42 Gene ID: 5119 Database links: Entrez Gene: 5119 Human Entrez Gene: 234852 Mouse Entrez Gene: 365024 Rat Omim: 164010 Human SwissProt: Q9HD42 Human SwissProt: Q921W0 Mouse Unigene: 589427 Human Unigene: 41596 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHMP1A) Polyclonal Antibody, Unconjugated (SL4207R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CHMP1A Polyclonal Antibody, Unconjugated(SL4207R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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