Product Name	SGPL1
Chinese Name	磷酸鞘氨醇裂解酶1抗体
Alias	FLJ13811; hSPL; KIAA1252; S1P; S1PL; SGPL 1; SGPL1; SGPL1_HUMAN; SP lyase; SP-lyase 1; Sphingosine 1 phosphate aldolase; Sphingosine 1 phosphate lyase 1; Sphingosine-1-phosphate aldolase; Sphingosine-1-phosphate lyase 1; SPL 1; SPL.
literatures	Specific References  (5)     |     SL4188R has been referenced in 5 publications. [IF=11.47] Choi, Won Hoon, et al. "Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation." Nature Communications 7 (2016).  WB ;  Human.   PubMed:26957043 [IF=5.853] Mili Minaduola. et al. The circadian clock sets a spatial–temporal window for recent thymic emigrants. IMMUNOL CELL BIOL. 2022 Sep;:  IF ;  Mouse.   PubMed:36030488 [IF=4.005] Karuppuchamy T et al. Sphingosine-1-Phosphate Lyase Inhibition Alters the S1P Gradient and Ameliorates Crohn's-Like Ileitis by Suppressing Thymocyte Maturation. Inflamm Bowel Dis. 2020 Jan 6;26(2):216-228.  IHC-P ;  Mouse.   PubMed:31807751 [IF=3.687] Lemos et al. Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders. (2018) Int.J.Mol.Sci. 19  FC/IHC ;  mouse.   PubMed:29757216 [IF=2.25] Zhang X et al. Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling.Biochem Cell Biol. 2018 Dec;96(6):742-751.  WB ;  Mouse&Human.   PubMed:29940125
Research Area	Tumour  Cardiovascular  immunology  Signal transduction  Lipoprotein  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	63kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SGPL1: 301-400/568
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. Tissue Specificity: Found in liver and kidney. Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. SWISS: O95470 Gene ID: 8879 Database links: Entrez Gene: 8879 Human Entrez Gene: 20397 Mouse Entrez Gene: 286896 Rat SwissProt: O95470 Human SwissProt: Q8R0X7 Mouse SwissProt: Q8CHN6 Rat Unigene: 499984 Human Unigene: 412319 Mouse Unigene: 431862 Mouse Unigene: 26953 Rat Omim: 603729 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (SL4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (SL4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (SL4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (SL4188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SGPL1) polyclonal Antibody, Unconjugated (SL4188R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: A549. Primary Antibody (green line): Rabbit Anti-SGPL1 antibody (SL4188R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: A549. Primary Antibody (green line): Rabbit Anti-SGPL1/FITC Conjugated antibody (SL4188R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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