Product Name	Synaptotagmin 1
Chinese Name	突触Binding protein1抗体
Alias	DKFZp781D2042; P65; SVP65; Synaptotagmin I; SYT1_HUMAN; Synaptotagmin II; Synaptotagmin IV; Synaptotagmin V; SYT 1; SYT; SYT1; SYT2; SYT3; SYT4; SYT5; SytI.
literatures	Specific References  (2)     |     SL4172R has been referenced in 2 publications. [IF=7.277] Wang, Yunfeng. et al. Repeated oxytocin prevents central sensitization by regulating synaptic plasticity via oxytocin receptor in a chronic migraine mouse model. J Headache Pain. 2021 Dec;22(1):1-16  WB ;  Mouse.   PubMed:34315403 [IF=3.244] Wang J et al. EphrinB/EphB signaling contributes to the synaptic plasticity of chronic migraine through NR2B phosphorylation. Neuroscience. 2020 Jan 21;428:178-191.  WB ;  Rat.   PubMed:31918010
Research Area	Tumour  immunology  Neurobiology  Signal transduction  Cell adhesion molecule  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Synaptotagmin 1: 165-260/422
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Function: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS). Subunit: Homotetramer (Probable). Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note=Synaptic vesicles and chromaffin granules. Similarity: Belongs to the synaptotagmin family. Contains 2 C2 domains. SWISS: P21579 Gene ID: 6857 Database links: Entrez Gene: 6857 Human Entrez Gene: 20979 Mouse Entrez Gene: 25716 Rat Omim: 185605 Human SwissProt: P21579 Human SwissProt: P46096 Mouse SwissProt: P21707 Rat Unigene: 310545 Human Unigene: 289702 Mouse Unigene: 216272 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebral cortex (Mouse) Lysate at 40 ug Cerebrum(Rat) Lysate at 40 ug Primary: Anti-Synaptotagmin 1 (SL4172R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 53kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Synaptotagmin 1/SYT1 Polyclonal Antibody, Unconjugated(SL4172R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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