Product Name	SYTL5
Chinese Name	突触样蛋白5亚型2抗体
Alias	synaptotagmin-like protein 5 isoform 2; synaptotagmin-like protein 5; SYTL5 protein; synaptotagmin-like 5; ENSMUSG00000054453; slp5; SYTL5.
Research Area	immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	81kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SYTL5: 601-700/730
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of this gene is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Function: May act as Rab effector protein and play a role in vesicle trafficking. Binds phospholipids. Subcellular Location: Membrane. Tissue Specificity: Highly expressed in placenta and liver. Similarity: Contains 2 C2 domains. Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain. SWISS: Q8TDW5 Gene ID: 94122 Database links: Entrez Gene: 94122 Human Entrez Gene: 236643 Mouse Entrez Gene: 302538 Rat SwissProt: Q8TDW5 Human SwissProt: Q80T23 Mouse SwissProt: Q812E4 Rat Unigene: 662334 Human Unigene: 246606 Mouse Unigene: 92480 Rat
Product Picture	Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human U251 cell lysates Lane 3: Human HeLa cell lysates Lane 4: Human A549 cell lysates Primary: Anti-SYTL5 (SL4145R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 75 kDa Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SYTL5 Polyclonal Antibody, Unconjugated(SL4145R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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