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Product Name Myoglobin Chinese Name 肌红蛋白抗体 Alias MYO; MB; MGC13548; PVALB; MYG_HUMAN. literatures Specific References (1) | SL41107R has been referenced in 1 publications.[IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212 WB ; Mouse.Research Area Tumour Cardiovascular Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 17kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen Purified Human Myoglobin (Natural Human Myoglobin protein) Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Myoglobin is a small heme containing protein responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
Function:
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Similarity:
Belongs to the globin family.
SWISS:
P02144
Gene ID:
4151
Database links:Entrez Gene: 4151 Human
Entrez Gene: 17189 Mouse
Omim: 160000 Human
SwissProt: P02144 Human
SwissProt: P04247 Mouse
Unigene: 517586 Human
Unigene: 404074 Mouse
Unigene: 40511 Rat
肌红蛋白存在于肌肉中,心肌中含量特别丰富,,有较高的组织特异性,是横纹肌的特异性标记物,但对Tumour的敏感性较差。主要用于横纹肌Tumour的诊断与鉴别诊断。少数恶性中胚叶混合瘤、睾丸畸胎瘤、肾母细胞瘤亦可见阳性表达。Product Picture Sample:
Human Myoglobin Protein at 100 ng
Muscle (Mouse) Lysate at 30 ug
Muscle (Rat) Lysate at 20 ug
Primary: Anti-Myoglobin (SL41107R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 14 kD
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Primary: Anti-Myoglobin (SL41107R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (SL41107R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (SL41107R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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