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Product Name P2X2 Chinese Name 三磷酸腺苷受体受体P2X2抗体 Alias ATP receptor; P2RX 2; P2RX2; P2RX2_HUMAN; P2X purinoceptor 2; P2X Receptor subunit 2; P2X2; P2X2a; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 2; Purinergic receptor P2X2 isoform A; Purinergic receptor P2X2 isoform B; Purinergic receptor P2X2 isoform C; Purinergic receptor P2X2 isoform D; Purinergic receptor P2X2 isoform H; Purinergic receptor P2X2 isoform I; MGC129601. Research Area Tumour Cell biology immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Guinea Pig, (predicted: Mouse, Rat, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 52kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ATP receptor: 251-350/471 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2013]
Function:
Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle.
Subunit:
Functional P2XRs are organized as homomeric and heteromeric trimers.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Similarity:
Belongs to the P2X receptor family.
SWISS:
Q9UBL9
Gene ID:
22953
Database links:Entrez Gene: 22953 Human
Entrez Gene: 231602 Mouse
Omim: 600844 Human
SwissProt: Q9UBL9 Human
Unigene: 258580 Human
Product Picture Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-P2X2 (SL4065R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:52 kD
Observed band size: 67 kD
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-P2X2 antibody (SL4065R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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