Product Name	Pancreatic Amylase
Chinese Name	胰淀粉酶抗体
Alias	Alpha amylase; Alpha amylase pancreatic precursor; AMY2; AMY2A; AMY2B; Amylase alpha 2A; Amylase alpha 2A pancreatic; Amylase alpha 2B; Amylase alpha 2B pancreatic; Amylase pancreatic alpha 2A; Amylase pancreatic alpha 2B; Pancreatic alpha amylase; AMYP_HUMAN.
literatures	Specific References  (1)     |     SL4030R has been referenced in 1 publications. [IF=9.685] Peng, Cheng. et al. MLKL signaling regulates macrophage polarization in acute pancreatitis through CXCL10. CELL DEATH DIS. 2023 Feb;14(2):1-14  IF ;  Mouse.   PubMed:36828808
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Pancreatic Amylase: 151-250/511
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa.Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). Subunit: Monomer. Subcellular Location: Secreted, extracellular space. Similarity: Belongs to the glycosyl hydrolase 13 family. SWISS: P04746 Gene ID: 279 Database links: Entrez Gene: 279 Human Entrez Gene: 280 Human Entrez Gene: 545562 Mouse Omim: 104650 Human Omim: 104660 Human SwissProt: P04746 Human SwissProt: P19961 Human SwissProt: P00688 Mouse Unigene: 484588 Human Unigene: 654437 Human     胰淀粉酶由胰腺以活性状态排入消化道，是最重要的水解碳水化合物的酶，和唾液腺分泌的淀粉酶一样都属于α-淀粉酶，作用于α-1，4糖苷键，对分支上的α-1，6糖苷键无作用，故又称淀粉内切酶，其作用的最适pH为6.9，可通过肾小球滤过，是唯一能在正常时于尿中出现的血浆酶。     人体的其他组织如卵巢、输卵管、肺、睾丸、精液、乳腺等的提取物中都发现有淀粉酶活性;血液、尿液、乳液中也含淀粉酶。血液淀粉酶中主要来自胰腺、唾液腺，尿液中淀粉酶则来自于血液.。当罹患胰腺疾病，或有胰腺外分泌功能障碍时都可引起其活性升高或降低，有助于胰腺疾病的诊断。尿淀粉酶水平波动较大，所以用血清淀粉酶检测为好，或两者同时测定。淀粉酶活性变化亦可见于某些非胰腺疾患。
Product Picture	Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (SL4030R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (SL4030R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 61 kD Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pancreatic Amylase) Polyclonal Antibody, Unconjugated (SL4030R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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