Product Name	ATG9B
Chinese Name	自噬相关蛋白9B抗体
Alias	APG9 like 2; APG9-like 2; APG9L2; Apgdc2; ATG9 autophagy related 9 homolog B; Atg9b; Autophagy related protein 9B; Autophagy-related protein 9B; eONE; Gm574; Nitric oxide synthase 3 overlapping antisense gene protein; Nitric oxide synthase 3-overlapping antisense gene protein; NOS3AS; Protein sONE; SONE; Apg912.
Research Area	Tumour  immunology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	94kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ATG9B: 351-450/923
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012] Function: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Subcellular Location: Cytoplasmic vesicle ?autophagosome membrane; Multi-pass membrane protein. Note: Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1 and ATG13. Tissue Specificity: Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial. Similarity: Belongs to the ATG9 family. SWISS: Q6EBV9 Gene ID: 285973 Database links: Entrez Gene: 285973 Human Entrez Gene: 213948 Mouse Entrez Gene: 499973 Rat Omim: 612205 Human SwissProt: Q674R7 Human SwissProt: Q6EBV9 Mouse Unigene: 707300 Human Unigene: 716846 Human Unigene: 332252 Mouse
Product Picture	Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATG9B Polyclonal Antibody, Unconjugated(SL4011R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATG9B Polyclonal Antibody, Unconjugated(SL4011R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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