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Product Name CCNG2 Chinese Name 周期素G2抗体 Alias CCNG2_HUMAN; Cyclin-G2; Cyclin G2; CyclinG2; CCNG 2; CCNG-2; literatures Specific References (1) | SL3822R has been referenced in 1 publications.[IF=4.2] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829. WB ; Mouse.Research Area Tumour immunology Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CCNG2: 151-250/344 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cyclin G2 may play a role in growth regulation and in negative regulation of cell cycle progesssion. Cyclin G2 expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase. It may also contribute in maintaining the quiescent state of differentiated cells. Cyclin G2 is similar to cyclin A in the cyclin box, although no kinase activity is detected.
Function:
May play a role in growth regulation and in negative regulation of cell cycle progesssion.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
High levels in cerebellum, thymus, spleen and prostate. Low levels in skeletal muscle.
Similarity:
Belongs to the cyclin family. Cyclin G subfamily.
SWISS:
Q16589
Gene ID:
901
Database links:Entrez Gene: 901 Human
Entrez Gene: 12452 Mouse
SwissProt: Q16589 Human
SwissProt: O08918 Mouse
Product Picture Sample: Brain(Rat) lysate at 30ug;
Primary: Anti-Cyclin G2 (SL3822R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD
May be formed of homo-dimerParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin G2) Polyclonal Antibody, Unconjugated (SL3822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:A431.
Primary Antibody (green line): Rabbit Anti-Cyclin G2 antibody (SL3822R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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