Product Name	Piwil2
Chinese Name	piwi样2蛋白抗体
Alias	PIWL2_HUMAN; Piwi-like protein 2; EC:3.1.26.-; HILI; CT80; mili; PIWIL1L; piwi like RNA-mediated gene silencing 2; Cancer/testis antigen 80 (CT80);
literatures	Specific References  (3)     |     SL3817R has been referenced in 3 publications. [IF=3.64] Shi, Ran-ran, et al. "The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1."?Cancer Immunology, Immunotherapy?(2013): 1-10.  WB ;  Human.   PubMed:24077852 [IF=3.49] Easley et al. Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model. (2015) Stem.Cell.Re. 14:347-55  IF(ICC) ;  Human.   PubMed:25863443 [IF=2.276] Tian Xiaohong. et al. 17-Allylamino-demethoxygeldanamycin Used Alone or in Combination with Sodium Orthovanadate Promotes Apoptosis and Inhibits Invasion of SH-SY5Y Cells by Modulating PIWIL2. Biomed Res Int. 2020;2020:7894712  WB ;  Human.   PubMed:10.1155/2020/7894712
Research Area	Tumour  Cell biology  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg /Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	107kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Piwil2/Mili: 885-973/973
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] Function: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Subunit: Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Subcellular Location: Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Tissue Specificity: Expressed in adult testis and in most tumors. Post-translational modifications: Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. Similarity: Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain. SWISS: Q8TC59 Gene ID: 55124 Database links: Entrez Gene: 55124 Human Entrez Gene: 57746 Mouse Entrez Gene: 306011 Rat Omim: 610312 Human SwissProt: Q8TC59 Human SwissProt: Q8CDG1 Mouse Unigene: 614809 Human Unigene: 85253 Mouse Unigene: 144968 Rat [PTM]Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
Product Picture	Sample: 293T Cell Lysate at 30 ug Primary: Anti- Piwil2 (SL3817R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107kD Sample: testis (Mouse) Lysate at 40 ug 293T Cell (Human) Lysate at 30 ug Primary: Anti- piwil2 (SL3817R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107 kD Sample: Lovo(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti-Piwil2 (SL3817R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/110 kD Observed band size: 110 kD Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug HepG2 (Human) Cell Lysate at 30 ug Lovo (Human) Cell Lysate at 30 ug 293T (Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug Primary: Anti-Piwil2 (SL3817R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100/110 kD Observed band size: 110 kD Blank control: Mouse spleen cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Piwil2 antibody(SL3817R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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