Product Name	Oct-4B/OCT4B-190
Chinese Name	胚胎Stem cells关键蛋白190抗体(N端)
Alias	Oct-4B; Oct4B; Oct 4B; Oct 4B-190; Oct4B-190; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Oct-3; NF-A3; Otf-3; Otf3; POU5F1.
literatures	Specific References  (8)     |     SL3816R has been referenced in 8 publications. [IF=3.899] Xiuxiu Liu. et al. PHF20 inhibition promotes apoptosis and cisplatin chemosensitivity via the OCT4‑p‑STAT3‑MCL1 signaling pathway in hypopharyngeal squamous cell carcinoma. Int J Oncol. 2021 Jul;59(1):1-14  WB ;  Human.   PubMed:33982773 [IF=3.166] Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106  WB ;  Human.   PubMed:26235742 [IF=2.48] Liu et al. OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation. (2017) Biomed.Res.Int. 2017:2756891  WB ;  Human.   PubMed:28473980 [IF=2.15] Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell?Cassociated genes in colorectal cancer."Medical Oncology 30.1 (2013): 1-11.e  WB ;  Human.   PubMed:23307247 [IF=2.02] Jiejuan Lai. et al. Comparison of the biological and functional characteristics of mesenchymal stem cells from intrahepatic and identical bone marrow. Stem Cell Res. 2021 Aug;55:102477  IF ;  Mouse.   PubMed:34343826 [IF=1.58] Liu, Lu, et al. "OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation." BioMed Research International 2017 (2017).  WB ;  Human.   PubMed:doi:10.1155/2017/2756891 [IF=1.45] Tanabe, A., et al. "Radioresistance of cancer stem‐like cell derived from canine tumours." Veterinary and Comparative Oncology (2014).  WB ;  Dog.   PubMed:25070729 [IF=1.29] Darabi, Shahram, et al. "Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure." In Vitro Cellular & Developmental Biology-Animal (2013): 1-15.  IF(ICC) ;  Rat.   PubMed:23771792
Research Area	Tumour  Cell biology  immunology  Stem cells  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=1μg /Test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21/39kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OCT4: 201-300/360
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]. SWISS: Q01860 Gene ID: 5460 Database links: Entrez Gene: 5460 Human Entrez Gene: 18999 Mouse Entrez Gene: 294562 Rat Omim: 164177 Human SwissProt: Q01860 Human SwissProt: P20263 Mouse Unigene: 249184 Human Unigene: 632482 Human Unigene: 646545 Human Unigene: 17031 Mouse Unigene: 161748 Rat OCT4B-190蛋白能够增强细胞在应激条件下抵抗凋亡的能力。OCT4基因在Stem cells的增殖、分化、应激反应、凋亡过程等多个生物学过程中发挥着重要的作用。
Product Picture	Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Cerebellum (Mouse) Lysate at 40 ug Lane 4: Testis (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Heart (Rat) Lysate at 40 ug Lane 7: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-Oct-4B/OCT4B-190 (SL3816R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (SL3816R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (SL3816R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (SL3816R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (SL3816R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).

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