Product Name	PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1
Chinese Name	丙酮酸脱氢酶激酶1抗体
Alias	PDHK1; mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDK 1; PDK-1; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1; PDK1_HUMAN.
literatures	Specific References  (2)     |     SL3788R has been referenced in 2 publications. [IF=2.906] Jinfeng Zhu et al. Expression and prognostic significance of pyruvate dehydrogenase kinase 1 in bladder urothelial carcinoma. Virchows Arch. 2020 Nov;477(5):637-649.  IHC ;  Human.   PubMed:32388719 [IF=2.705] Hu HJ et al. Regulating the Warburg effect on metabolic stress and myocardial fibrosis remodeling and atrial intracardiac waveform activity induced by atrial fibrillation. Biochem Biophys Res Commun. 2019 Aug 27;516(3):653-660.  WB ;  beagle canine.   PubMed:31242971
Research Area	Tumour  immunology  Growth factors and hormones  Kinases and Phosphatases  Synthesis and Degradation  glycoprotein  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PDK1: 51-150/436
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013] Function: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Expressed predominantly in the heart. Similarity: Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. SWISS: Q15118 Gene ID: 5163 Database links: Entrez Gene: 5163 Human Entrez Gene: 228026 Mouse Entrez Gene: 116551 Rat Omim: 602524 Human SwissProt: Q15118 Human SwissProt: Q8BFP9 Mouse SwissProt: Q63065 Rat Unigene: 470633 Human Unigene: 34411 Mouse Unigene: 11185 Rat
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1(SL3788R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDK1/Mitochondrial Pyruvate dehydrogenase kinase 1) Polyclonal Antibody, Unconjugated (SL3788R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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