TEL: +86 571 56623320 EMAIL: [email protected]
Product Name phospho-Crkl (Tyr251) Chinese Name 磷酸化接头蛋白CrkL抗体 Alias Crk L; Crk like protein; Crkol; HGNC:2363; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; V crk sarcoma virus CT10 oncogene homolog avian like; CRKL_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251: CA(p-Y)D Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.
Function:
May mediate the transduction of intracellular signals.
Subunit:
Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4.
Similarity:
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
SWISS:
P46109
Gene ID:
1399
Database links:Entrez Gene: 1399 Human
Entrez Gene: 12929 Mouse
Omim: 602007 Human
SwissProt: P46109 Human
SwissProt: P47941 Mouse
Unigene: 5613 Human
Unigene: 21048 Mouse
Unigene: 451241 Mouse
接头蛋白CrkL是Crk家族成员之一,CRKL为具有SH2及SH3结构的连接蛋白,CrkL参与多种Signal transduction途径调节细胞运动、增殖及分化,在各种组织中广泛表达,在多种信号途径中起重要作用。Product Picture Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Crkl (Tyr251)) Polyclonal Antibody, Unconjugated (SL3738R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode