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Product Name TCTP Chinese Name 翻译控制Tumour蛋白抗体 Alias TPT1-like protein; TPT1L + TCTP; TPT1L_HUMAN; FLJ27337; Fortilin; Histamine releasing factor; Histamine-releasing factor; HRF; Immunoglobulin E-dependent; p02; p23; TCTP; TCTP_HUMAN; TPT1; Translationally controlled tumor protein; Translationally-controlled tumor protein; Tumor protein translationally controlled 1; tumor protein translationally-controlled 1; tumor protein, translationally-controlled 1. literatures Specific References (1) | SL3578R has been referenced in 1 publications.[IF=1.26] Wu et al. Upregulation of TCTP expression in human skin squamous cell carcinoma increases tumor cell viability through anti-apoptotic action of the protein. (2012) Exp.Ther.Med. 3:437-442 IHC-P ; Human.Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 20kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TCTP: 85-172/172 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
Function:
Involved in calcium binding and microtubule stabilization.
Subunit:
Interacts with STEAP3.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
Similarity:
Belongs to the TCTP family.
SWISS:
P13693
Gene ID:
7178
Database links:Entrez Gene: 7178 Human
Entrez Gene: 22070 Mouse
Omim: 600763 Human
SwissProt: P13693 Human
SwissProt: P63028 Mouse
Unigene: 374596 Human
Unigene: 296922 Mouse
Unigene: 297482 Mouse
Unigene: 36610 Rat
翻译控制Tumour蛋白最初发现于Tumour组织,后来又从其他组织中相继分离出来,是许多动物Tumour细胞中存在的一类较丰富的蛋白,TCTP参与钙离子结合和微管的稳定,是一类广泛存在于动物、Botany及酵母中的、在序列上高度保守且具有很高表达量的蛋白家族。Product Picture Sample:
Jurkat(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-TCTP (SL3578R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 22 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TCTP) Polyclonal Antibody, Unconjugated (SL3578R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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