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Product Name DMT1 Chinese Name 金属离子转运体1抗体 Alias DCT 1; DCT1; Divalent metal transporter 1; DMT 1; Natural resistance associated macrophage protein 2; NRAMP 2; NRAMP2; SLC11A2; NRAM2_HUMAN. literatures Specific References (8) | SL3577R has been referenced in 8 publications.Research Area Tumour immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 62kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DMT1: 251-350/568 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail DMT1 is a major transporter of ferrous iron from the intestinal lumen. DMT1 belongs to the family of NRAMP metal ion transporters which function in diverse organisms from bacteria to human. DMT1 (NRAMP2) transports metal ions at the plasma membrane of cells of both the duodenum and in peripheral tissues, and defective DMT1 causes anaemia.
Function:
Actin-bundling protein. May function in mitogen-activated protein kinase pathway.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Heart, brain, lung, skeletal muscle, and kidney.
Post-translational modifications:
Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.
Similarity:
Belongs to the villin/gelsolin family.
Contains 1 HP (headpiece) domain.
SWISS:
P49281
Gene ID:
4891
Database links:Entrez Gene: 4891 Human
Entrez Gene: 18174 Mouse
Omim: 600523 Human
SwissProt: P49281 Human
SwissProt: P49282 Mouse
Unigene: 505545 Human
Unigene: 234608 Mouse
Product Picture Sample:
Adrenal glands (Mouse) Lysate at 40 ug
Primary: Anti-DMT1 (SL3577R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (SL3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Divalent metal transporter 1; DMT1) Polyclonal Antibody, Unconjugated (SL3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (SL3577R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-DMT1 antibody (SL3577)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-DMT1 antibody (SL3577R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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