Product Name	Phospho-PPIG (Ser376)
Chinese Name	磷酸化亲环蛋白（亲环素）PPIG抗体
Alias	CARS cyclophilin; CARS Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Rotamase G; RotamaseG; PPIG_HUMAN.
Product Type	Phosphorylated anti
Research Area	Cell biology  immunology  Chromatin and nuclear signals
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	89kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human PPIG around the phosphorylation site of Ser376: VS(p-S)GE
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PPIG belongs to the the peptidyl-prolyl cis/trans isomerase protein family (PPIases). PPIases accelerate the folding of proteins. PPIG catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is distributed in nuclear speckles, a nuclear compartment rich in splicing factors, and colocalizes with the splicing factors SC35 and pinin, suggesting a role in the regulation of pre-mRNA splicing. Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing. Subunit: Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II. Subcellular Location: Nucleus matrix. Nucleus speckle. Note=Colocalizes with RNA splicing factors at nuclear speckles. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 PPIase cyclophilin-type domain. SWISS: Q13427 Gene ID: 9360 Database links: Entrez Gene: 9360 Human Entrez Gene: 228005 Mouse Entrez Gene: 83624 Rat Omim: 606093 Human SwissProt: Q13427 Human SwissProt: A2AR02 Mouse SwissProt: O55035 Rat Unigene: 727580 Human Unigene: 11815 Mouse Unigene: 474951 Mouse Unigene: 10898 Rat 肽基脯氨酰基顺反异构酶，广泛存在于各种组织和器官。根据对免疫抑制剂的敏感性，分属三个独立的家族：亲环蛋白PPIG、FK506Binding protein和细蛋白。能够加速自身折叠和体内新生肽链折叠及变性蛋白质的体外折叠，主要分布于内质网。
Product Picture	Sample: Testis (Mouse) Lysate at 40 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-PPIG (Ser376) (SL3461R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 89 kD Observed band size: 110 kD Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPIG (Ser376)) Polyclonal Antibody, Unconjugated (SL3461R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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