Product Name	Phospho-eNOS (Ser1177)
Chinese Name	磷酸化一氧化氮合成酶3（内皮型）抗体
Alias	eNOS (phospho S1177); eNOS(phospho S1177); p-eNOS (phospho S1177); eNOS (Phospho-Ser1177); cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.
literatures	Specific References  (12)     |     SL3447R has been referenced in 12 publications. [IF=8.101] Sol Guerra-Ojeda. et al. Cerium dioxide nanoparticles modulate antioxidant defences and change vascular response in the human saphenous vein. FREE RADICAL BIO MED. 2022 Nov;193:694  WB ;  Human.   PubMed:36402438 [IF=5.391] Kim DY et al. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175. FASEB J. 2019 Jun 6:fj201802516RR.  WB ;  Human.   PubMed:31170000 [IF=5.195] Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476  WB ;  Mouse.   PubMed:35724747 [IF=5.011] Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436  WB ;  Human.   PubMed:36754152 [IF=3.82] Ikemura, S., et al. "Preventive effects of the anti-vasospasm agent via the regulation of the Rho-kinase pathway on the development of steroid-induced osteonecrosis in rabbits." Bone (2013).  WB ;  Rabbit.   PubMed:23313282 [IF=3.777] Mengmeng Shen. et al. Erbu Zhuyu decoction improves endometrial angiogenesis via uterine natural killer cells and the PI3K/Akt/eNOS pathway a mouse model of embryo implantation dysfunction. AM J REPROD IMMUNOL. 2022 Nov;:  WB, IHC ;  Mouse.   PubMed:36327113 [IF=3.457] Gao Y et al. Ginsenoside Re inhibits vascular neointimal hyperplasia in balloon-injured carotid arteries through activating the eNOS/NO/cGMP pathway in rats Y Gao, CY Gao, P Zhu, SF Xu, YM Luo, J Deng… - Biomedicine & …, 2018Biomed Pharmacother. 2018 Oct;106:1091-1097.  IHC-P ;  Rat.   PubMed:30119175 [IF=2.85] Chen, I., et al. "Statins ameliorate pulmonary hypertension secondary to left ventricular dysfunction through the Rho‐kinase pathway and NADPH oxidase." Pediatric Pulmonology (2016).  IHC-P ;  Rat.   PubMed:28029743 [IF=2.171] Zhang Q et al. Sulfur dioxide induces vascular relaxation through PI3K/Akt/eNOS and NO/cGMP signaling pathways in rats. Hum Exp Toxicol. 2020 Mar 10:960327120911428.  WB ;  rat.   PubMed:32153200 [IF=1.823] Xinxin Li. et al. Nebivolol Alleviates Vascular Endothelial Insulin Resistance by Inhibiting Endoplasmic Reticulum Stress. INT HEART J. 2023 Mar 15  WB ;  Human.   PubMed:36927931 [IF=1.28] Yin, Yaling, et al. "Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO‑cGMP signaling pathway." Experimental and therapeutic medicine 8.1 (2014): 38-48.  IF(ICC) ;  Human.   PubMed:24944594 [IF=1.2] Özel F et al. Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300.  IHC ;  rat.   PubMed:32127153
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases  Synthesis and Degradation  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg /Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	133kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser1177: TQ(p-S)FS
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Subunit: Homodimer. Interacts with NOSIP and NOSTRIN. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Tissue Specificity: Platelets, placenta, liver and kidney. Post-translational modifications: Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. Similarity: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. SWISS: P70313 Gene ID: 18127 Database links: Entrez Gene: 287024 Cow Entrez Gene: 403784 Dog Entrez Gene: 4846 Human Entrez Gene: 18127 Mouse Entrez Gene: 397557 Pig Entrez Gene: 24600 Rat Omim: 163729 Human SwissProt: P29473 Cow Synthesis and Degradation（Synthesis and Degradation) 催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。此抗体识别分子量为135kDa的内皮型一氧化氮合成的酶。
Product Picture	Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti- p-eNOS (Ser1177) (SL3447R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kD Observed band size: 133 kD The blue histogram is unstained cells(HepG 2 cells). The Orange histogram is cells stained with Rabbit IgG/FITC (SL0295P-FITC) The green histogram is cells stained with Rabbit Anti-Phospho-eNOS (Ser1177)/FITC Conjugated antibody (SL3447R-FITC). Isotype control: Cell lines treated with Rabbit IgG/FITC (SL0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 100 μl 1 X PBS containing 0.5% BSA.

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