Product Name	Phospho-PFKFB3/PFK2 (Ser467)
Chinese Name	磷酸化果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体
Alias	6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; IPFK2; PFK/FBPase 3; PFK-2; PFK2; Renal carcinoma antigen NY REN 56; uPFK 2; PFKFB3; F263_HUMAN.
literatures	Specific References  (5)     |     SL3331R has been referenced in 5 publications. [IF=4.6] Fu, Wen, et al. "Bioenergetic mechanisms in astrocytes may contribute to amyloid plaque deposition and toxicity." Journal of Biological Chemistry (2015): jbc-M114.  Human.   PubMed:25814669 [IF=4.129] Jiang, Bin. et al. Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery. Obes Surg. 2022 Jan;:1-11  WB ;  Rat.   PubMed:35080701 [IF=3.546] Yi,et al.The EMT-related transcription factor snail up-regulates FAPα in malignant melanoma cells.(2018) Experimental Cell Research. 364:160-167.  IHC-P ;  Human.   PubMed:29410133 [IF=3.231] Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488  WB ;  Chicken.   PubMed:36230229 [IF=0] Robinson,et al.Role of reactive oxygen species in modulating carbohydrate metabolism in acute myeloid leukaemia. Cardiff University, 2017.  WB ;  Human.   PubMed:doi:10.5353/th_b5774074
Product Type	Phosphorylated anti
Research Area	Cell biology  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human PFK2 around the phosphorylation site of Ser467: LA(p-S)PE
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] Function: Synthesis and degradation of fructose 2,6-bisphosphate. Tissue Specificity: Ubiquitous. Similarity: In the C-terminal section; belongs to the phosphoglycerate mutase family. SWISS: Q16875 Gene ID: 5209 Database links: Entrez Gene: 5209 Human Entrez Gene: 170768 Mouse Entrez Gene: 24640 Rat Omim: 605319 Human SwissProt: Q16875 Human SwissProt: P25114 Rat Unigene: 444304 Human Unigene: 44844 Rat
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug  Primary: Anti- Phospho-PFKFB3/PFK2 (Ser467) (SL3331R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3 PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (SL3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (SL3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PFKFB3/PFK2 (Ser467)) Polyclonal Antibody, Unconjugated (SL3331R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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