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Rabbit Anti-Strep-Tag II antibody
Rabbit Anti-Strep-Tag II antibody
Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG.
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Details

Product Name Strep-Tag II
Chinese Name Strep-Tag II(标签)抗体
Alias Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG.  
Research Area Cell biology  
Immunogen Species Rabbit
Clonality Polyclonal
Applications WB=1:500-2000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Cellular localization Secretory protein 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated Strep-Tag 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

SWISS:
N/A

Gene ID:
N/A

Product Picture
Sample:
Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-Strep-Tag II (SL33016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

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