Product Name	Phospho-GCN2 (Thr899)
Chinese Name	磷酸化蛋白激酶GCN2蛋白抗体
Alias	GCN2 (phospho T899); p-GCN2 (phospho T899); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2.
literatures	Specific References  (2)     |     SL3155R has been referenced in 2 publications. [IF=25.269] Changzheng Li. et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. CELL STEM CELL. 2022 Jul;29:1119  WB ;  Mouse.   PubMed:35803229 [IF=5.58] Lehman, Stacey L., Sandra Ryeom, and Constantinos Koumenis. "Signaling through alternative Integrated Stress Response pathways compensates for GCN2 loss in a mouse model of soft tissue sarcoma." Scientific Reports 5 (2015).  WB ;  Mouse.   PubMed:26123823
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Neurobiology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	189kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899: HL(p-T)GM
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. Function: Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. Tissue Specificity: Widely expressed. Post-translational modifications: Autophosphorylated on threonine residues. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. SWISS: Q9P2K8 Gene ID: 440275 Database links: Entrez Gene: 440275 Human Entrez Gene: 27103 Mouse Entrez Gene: 114859 Rat Omim: 609280 Human SwissProt: Q9P2K8 Human SwissProt: Q9QZ05 Mouse Unigene: 656673 Human Unigene: 217616 Mouse Unigene: 8333 Rat GCN2蛋白的作用；可能是将短期记忆变成长期记忆的一个主控调节因子， GCN2蛋白在长期记忆形成中功能的发现将可能帮助研究人员开发出提高记忆力丧失的病人提高记忆力。
Product Picture	Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (SL3155R) Dilution:1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-Phospho-GCN2 (Thr899) antibody (SL3155R) Dilution:1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (SL3155R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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