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Product Name Phospho-Bcr (Tyr177) Chinese Name 磷酸化Tlymphocyte受体抗体 Alias Bcr (phospho Y177); Bcr(Phospho-Tyr177); ALL; BCR 1; BCR/ABL FUSION GENE INCLUDED; BCR/FGFR1 FUSION GENE INCLUDED; BCR/PDGFRA FUSION GENE INCLUDED; BCR1; breakpoint cluster region; CML; PHL; BCR_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Signal transduction The cell membrane受体 Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Pig, Rabbit, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 143kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesisedphosphopeptide derived from human Bcr around the phosphorylation site of Tyr177: PF(p-Y)VN Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Function:
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Subunit:
Homotetramer. Interacts with PDZK1. May interact with CCPG1. Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK.
Post-translational modifications:
Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
DISEASE:
Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
Similarity:
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
SWISS:
P11274
Gene ID:
613
Database links:Entrez Gene: 613 Human
Omim: 151410 Human
SwissProt: P11274 Human
Unigene: 517461 Human
Unigene: 715409 Human
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