Product Name	Phospho-CD19 (Tyr531)
Chinese Name	磷酸化CD19抗体
Alias	CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Signal transduction  Cell Surface Molecule  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	59kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531: DS(p-Y)EN <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CD19 is a transmembrane glycoprotein that is delectively expressed on the cell surface of B-lymphocytes,where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways.Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Function: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Subunit: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. DISEASE: Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P15391 Gene ID: 930 Database links: Entrez Gene: 930 Human Entrez Gene: 12478 Mouse Entrez Gene: 365367 Rat Omim: 107265 Human SwissProt: P15391 Human SwissProt: P25918 Mouse Unigene: 652262 Human Unigene: 4360 Mouse CD19是一种质膜蛋白质，参与信号传导作用。表达与前B细胞和成熟的BThe cell membrane表面，与B细胞的活化调节和发育调节相关，在T细胞和正常粒细胞上无表达。此抗体可以特异性识别CD19，主要用于标记正常B细胞及Tumour性B细胞。
Product Picture	Sample: Lane 1: Human Raji cell lysates Lane 2: Human K562 cell lysates Primary: Anti-Phospho-CD19 (Tyr531) (SL3065R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 105 kD Blank control (blue line): HL60 cells (fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD19 antibody (SL3065R),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. phosphopeptide non phosphopeptide

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