Product Name	phospho-arfaptin 2 (Ser260)
Chinese Name	磷酸化ADP核糖基化因子Binding protein2抗体
Alias	arfaptin 2 (phospho Ser260); arfaptin 2 (phospho S260); ADP ribosylation factor interacting protein 2; Arfaptin 2; ARFIP2; Partner of RAC1; POR1; ARFP2_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Neurobiology  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Cow, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human arfaptin 2 around the phosphorylation site of Ser260: LE(p-S)AQ
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes. Function: Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. Subunit: Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level. Similarity: Contains 1 AH domain. SWISS: P53365 Gene ID: 23647 Database links: Entrez Gene: 23647 Human Entrez Gene: 76932 Mouse Entrez Gene: 293344 Rat Omim: 601638 Human SwissProt: P53365 Human SwissProt: Q8K221 Mouse SwissProt: Q6AY65 Rat Unigene: 595146 Human Unigene: 75139 Human Unigene: 41637 Mouse Unigene: 73878 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-arfaptin 2 (Ser260)) Polyclonal Antibody, Unconjugated (SL3006R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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