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Product Name prox1 Chinese Name prox-1抗体 Alias Homeobox prospero like protein; Homeobox prospero like protein PROX1; Prospero related homeobox 1;prox-1; PROX 1. literatures Specific References (3) | SL2774R has been referenced in 3 publications.[IF=1.918] Gao,et al.High Expression of Prospero-Related Homeobox-1 (PROX1) Is Associated With Poor Prognosis in Patients With Salivary Adenoid Cystic Carcinoma.(2018) Journal of Oral and Maxillofacial Surgery. :. IHC-P ; Human.[IF=1.61] Usui, Tatsuya, et al. "Establishment of a novel three‐dimensional primary culture model for hippocampal neurogenesis." Physiological Reports 5.12 (2017): e13318. IF(IHC-P) ; Mouse.[IF=1.347] Teng,et al.Anti-inflammatory effect of tranexamic acid against trauma-hemorrhagic shock-induced acute lung injury in rats.(2018) Experimental Animals. :. WB ; Rat.Research Area Cardiovascular immunology Neurobiology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:2000-5000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 81kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human prox-1: 151-250/737 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Prox1 is a prospero-related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and the development of postmitotic undifferentiated young neurons.
Subcellular Location:
Nucleus.
Tissue Specificity:
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
SWISS:
Q92786
Gene ID:
5629
Database links:Entrez Gene: 395802 Chicken
Entrez Gene: 5629 Human
Entrez Gene: 19130 Mouse
Entrez Gene: 399348 Xenopus laevis
Entrez Gene: 30679 Zebrafish
Omim: 601546 Human
SwissProt: Q91018 Chicken
SwissProt: Q92786 Human
SwissProt: P48437 Mouse
Unigene: 741808 Human
Unigene: 744931 Human
Unigene: 132579 Mouse
Unigene: 392678 Mouse
Unigene: 26557 Rat
prox-1主要表达于晶状体、心脏、肝脏、胰腺和神经系统等的非endothelial cells,在成人正常组织、胚胎淋巴管endothelial cells或Tumour组织的淋巴endothelial cells上也可检测到。移除prox-1基因后,胚胎发育时原始静脉endothelial cells生成淋巴管过程被阻断,表明prox-1是淋巴管生成最基本的调控因子.Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-prox1 Polyclonal Antibody, Unconjugated(SL2774R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-prox1 antibody (SL2774R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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