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Product Name IL-1R1 Chinese Name 白介素1受体1抗体 Alias Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha type 1; Interleukin 1 receptor antagonist protein (IRAP); Interleukin 1 receptor type I; Interleukin receptor 1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; P80; IL1 Receptor I; IL-1 Receptor I; Interleukin-1 receptor type 1, soluble form; IL-1 sRI. literatures Specific References (3) | SL2594R has been referenced in 3 publications.[IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse.[IF=5.682] Pan, Jingrui. et al. Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6. MOL NEUROBIOL. 2023 Jan;:1-18 WB,IF ; Rat.[IF=4.427] Zhang H et al. Aluminum trichloride-induced hippocampal inflammatory lesions are associated with IL-1β-activated IL-1 signaling pathway in developing rats. Chemosphere. 2018 Jul;203:170-178. WB ; Rat.Research Area Cardiovascular immunology Stem cells lymphocyte t-lymphocyte cell factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 63kDa Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IL-1R1: 481-569/569 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq].
Function:
Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.
Subunit:
The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Cell membrane (Probable). Secreted.
Post-translational modifications:
A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding).
Similarity:
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
SWISS:
P14778
Gene ID:
3554
Database links:Entrez Gene: 3554 Human
Entrez Gene: 16177 Mouse
Omim: 147810 Human
SwissProt: P14778 Human
SwissProt: P13504 Mouse
Unigene: 516249 Human
Unigene: 701982 Human
Unigene: 896 Mouse
Unigene: 9758 Rat
IL-1RⅠ属于新的信号受体家族TollSignal transduction通路蛋白,IL-1RⅠ在免疫和炎症反应的研究中占重要地位,是在很多感染 、炎症性致病机制中起决定性作用的cell factor,几乎可作用于全身各种细胞,IL-1 与IL-1R1的结合可导致大量与免疫反应和炎症相关基因的表达 ,包括cell factor、cell factor受体、急性期反应物、生长因子、胞外基质、粘附分子等。IL-1R1/NF-κB信号通路是机体免疫反应中十分重要的组成部分。白介素1受体I型 Interleukin 1 receptor I(IL-1RI)Product Picture Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-IL-1R1 (SL2594R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-IL-1R1 antibody (SL2594R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE; Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol overnight at 4℃ . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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