Product Name	Caspase 3 precursor
Chinese Name	半胱天冬酶-3酶原抗体
Alias	Caspase 3 precursor; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
literatures	Specific References  (13)     |     SL2593R has been referenced in 13 publications. [IF=12.479] Jun Jiang. et al. Nano-enabled photosynthesis in tumours to activate lipid peroxidation for overcoming cancer resistances. BIOMATERIALS. 2022 Jun;285:121561  IHC ;  Mouse.   PubMed:35537337 [IF=6.691] Barzegar-fallah, Anita. et al. Serotonin type-3 receptor antagonists selectively kill melanoma cells through classical apoptosis, microtubule depolymerisation, ERK activation, and NF-κB downregulation. 2021 Oct 15  WB ;  Human,Mouse.   PubMed:34654991 [IF=5.74] Duan, Xiaoxu, et al. "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic factor Bcl− 2 in human keratinocytes." Free Radical Biology and Medicine(2016).  WB ;  Human.   PubMed:26878773 [IF=4.486] Mingying Han. et al. Verapamil inhibits early acute liver failure through suppressing the NLRP3 inflammasome pathway. 2021 May 24  WB ;  Mouse.   PubMed:34031983 [IF=3.85] Luo, Xiaoming, et al. "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." European Journal of Pharmaceutics and Biopharmaceutics (2015).  IHC-P ;  Mouse.   PubMed:26523356 [IF=3.845] Zhu Z et al. Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy. Front Pharmacol. 2020 Feb 21;11:94.  WB ;  Rat.   PubMed:32153406 [IF=3.738] Salman, Giray. et al. Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line. 2021 Sep 21  WB ;  human.   PubMed:34546492 [IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68  WB ;  Rat.   PubMed:34004234 [IF=2.406] Simsek, Aysel. et al. Anti-proliferative and apoptotic effects of green synthesized silver nanoparticles using Lavandula angustifolia on human glioblastoma cells. 3 Biotech. 2021 Aug;11(8):1-10  WB ;  Human.   PubMed:34367866 [IF=2.23] Wang et al. Ginsenoside Rd inhibits apoptosis following spinal cord ischemia/reperfusion injury. (2014) Neural.Regen.Res. 9:1678-87  IHC ;  Rat.   PubMed:25374589 [IF=2.21] Can Sezer. et al. Neuroprotective Effects of Milrinone on Acute Traumatic Brain İnjury. WORLD NEUROSURG. 2022 Nov;:  IHC ;  Mouse.   PubMed:36403936 [IF=1.834] Uygar Saçık. et al. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. BIOTECH HISTOCHEM. 2022 Aug 23  IHC ;  Rat.   PubMed:35996879 [IF=1.06] Zhang, Xiaolin, and Hao Yu. "Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016)." Iranian Journal of Pharmaceutical Research (2016).  WB ;  Rat.   PubMed:27642320
Research Area	Cell biology  Neurobiology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Caspase 3 precursor: 11-120/277
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modifications: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Similarity: Belongs to the peptidase C14A family. SWISS: P55213 Gene ID: 836 Database links: Entrez Gene: 836 Human Entrez Gene: 12367 Mouse Entrez Gene: 397244 Pig Entrez Gene: 100008840 Rabbit Entrez Gene: 25402 Rat Omim: 600636 Human SwissProt: P42574 Human SwissProt: P70677 Mouse SwissProt: Q95ND5 Pig SwissProt: Q8MJC3 Rabbit SwissProt: P55213 Rat Unigene: 141125 Human Unigene: 34405 Mouse Unigene: 10562 Rat
Product Picture	Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor  (SL2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor (SL2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor  (SL2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Caspase 3 precursor  (SL2593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD Sample: Lane1: Brain (Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-pro-caspase-3 (SL2593R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1:3000 dilution; Predicted band size : 28kD Observed band size : 30kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-pro-caspase-3 Polyclonal Antibody, Unconjugated(SL2593R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Caspase 3 precursor antibody (SL2593R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test. Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-Caspase 3 precursor antibody (SL2593R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Caspase 3 precursor antibody (SL2593R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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