Product Name	LAMP3
Chinese Name	溶酶体相关膜蛋白3抗体
Alias	DC-lysosome-associated membrane glycoprotein; CD208; CD208 antigen; DC LAMP; DCLAMP; DC-LAMP; LAMP; LAMP 3; LAMP-3; LAMP3; Lysosomal associated membrane protein 3; Protein TSC403; TSC403; DC lysosome associated membrane glycoprotein; LAMP3_HUMAN; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3.
Research Area	Tumour  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LAMP-3: 301-416/416
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	LAMP3 might change the lysosome function after the transfer of peptide MHC class II molecules to the surface of dendritic cells. It's over expression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer. Expressed in lymphoid organs and dendritic cells and lung. Up regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. Function: Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. Subcellular Location: Lysosome membrane. Tissue Specificity: Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. Similarity: Belongs to the LAMP family. SWISS: Q9UQV4 Gene ID: 27074 Database links: Entrez Gene: 27074 Human Omim: 605883 Human SwissProt: Q9UQV4 Human Unigene: 518448 Human
Product Picture	Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti- LAMP3 (SL2556R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-LAMP3 antibody (SL2556R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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