Product Name	GLUT12
Chinese Name	葡萄糖Transporter12抗体
Alias	GLUT-12; GTR12_HUMAN; Glucose transporter type 12; Glucose transporter type 12; GLUT 12; GLUT8; SLC2A12; Solute carrier family 2 (facilitated glucose transporter) member 12; Solute carrier family 2 facilitated glucose transporter member 12; Solute carrier family 2 member 12.
literatures	Specific References  (8)     |     SL2540R has been referenced in 8 publications. [IF=11.062] Xiong Ying. et al. SLC2A12 of SLC2 Gene Family in Bird Provides Functional Compensation for the Loss of SLC2A4 Gene in Other Vertebrates. Mol Biol Evol. 2020 Nov;:  WB,IHC ;  Sparrow.   PubMed:33316072 [IF=4.586] Gil-Iturbe E et al. GLUT12 Expression in Brain of Mouse Models of Alzheimer's Disease. Mol Neurobiol. 2019 Aug 31.  WB ;  Mouse.   PubMed:31473905 [IF=4.556] Yoichi Chiba. et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium. Int J Mol Sci. 2020 Jan;21(19):7230  IHC ;  Human.   PubMed:33008107 [IF=4.232] Paweł Jan Stanirowski. et al. Placental expression of glucose transporters GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in pregnancies complicated by gestational and type 1 diabetes mellitus.. 2021 Sep 23  IHC ;  human.   PubMed:34555239 [IF=4.17] Pujol-Gimenez, Jonai, et al. "Expression of the Glucose Transporter GLUT12 in Alzheimers Disease Patients." Journal of Alzheimers Disease (2014).  WB ;  Human&Rat.   PubMed:24820014 [IF=3.455] Gil-Iturbe et al. Effect of aging and obesity on GLUT12 expression in small intestine, adipose tissue, muscle and kidney, and its regulation by docosahexaenoic acid and exercise in mice. Appl Physiol Nutr Metab. 2020 Mar 16.  WB ;  human.   PubMed:32176854 [IF=3.05] Murakami, Ryuta, et al. "Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells." Histochemistry and Cell Biology (2016):1-6  IHC-F ;  Mouse.   PubMed:27160096 [IF=2.64] Pujol-Gimenez, Jonai, et al. "Expression of the Glucose Transporter GLUT12 in Alzheimers Disease Patients." Journal of Alzheimers Disease (2014).  IHC-P ;  Human.   PubMed:25561978
Research Area	immunology  Transporter
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GLUT12: 251-350/617 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GLUT12 belongs to a family of transporters that catalyze the uptake of sugars through facilitated diffusion. Thirteen different types of glucose/fructose transport carrier proteins designated as Glut 1-13 facilitate glucose/fructose transport across the cell membrane. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Function: Facilitative glucose transporter. Subcellular Location: Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region. Note=Localizes primarily perinuclear region in the absence of insulin. Tissue Specificity: Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney. Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. SWISS: Q8TD20 Gene ID: 154091 Database links: Entrez Gene: 154091?/span>Human Entrez Gene: 353169?/span>Mouse Entrez Gene: 308028?/span>Rat Omim: 610372?/span>Human SwissProt: Q8TD20?/span>Human SwissProt: Q8BFW9?/span>Mouse Unigene: 486508?/span>Human Unigene: 473705?/span>Mouse
Product Picture	Sample: Heart (Rat) Lysate at 40 ug Primary: Anti- GLUT12 (SL2540R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (SL2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (SL2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Glut12 Polyclonal Antibody, Unconjugated(SL2540R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (SL2540R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. A-549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (SL2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (SL2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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