Product Name	MuRF1
Chinese Name	肌肉细胞特异性Ubiquitin蛋白连接酶1抗体
Alias	MTrim63; uRF 1; MuRF-1; Muscle-specific RING finger protein 1; Muscle-specific RING finger protein 1; E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; MURF1; MURF 1; MURF2; RNF28; SMRZ; Iris ring finger protein; Muscle specific ring finger protein 2; Ring finger protein 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRIM 63; TRIM63; Tripartite motif containing 63; Tripartite motif containing protein 63; Ubiquitin ligase TRIM63.
literatures	Specific References  (13)     |     SL2539R has been referenced in 13 publications. [IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858  WB ;  Mouse.   PubMed:37039469 [IF=7.971] Dvir Netanely. et al. Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns. Oncogene. 2021 Feb;:1-14  IHC ;  Human.   PubMed:33564068 [IF=6.228] Xuzhu Lin. et al. Undercarboxylated osteocalcin and ibandronate combination ameliorates hindlimb immobilization-induced muscle wasting. J PHYSIOL-LONDON. 2023 Apr;:  WB ;  Human.   PubMed:36999349 [IF=6.208] Sheng-Hua Wu. et al. Spinal Irisin Gene Delivery Attenuates Burn Injury-Induced Muscle Atrophy by Promoting Axonal Myelination and Innervation of Neuromuscular Junctions. INT J MOL SCI. 2022 Jan;23(24):15899  WB ;  Rat.   PubMed:36555538 [IF=5.846] Shuai Wang. et al. Effect of optimized new Shengmai powder on exercise tolerance in rats with heart failure by regulating the ubiquitin-proteasome signaling pathway. FRONT CARDIOVASC MED. 2023; 10: 1168341  WB ;  Rat.   PubMed:37288261 [IF=5.719] Piao Wang. et al. Monotropein Improves Dexamethasone-Induced Muscle Atrophy via the AKT/mTOR/FOXO3a Signaling Pathways. NUTRIENTS. 2022 Jan;14(9):1859  WB ;  Mouse.   PubMed:35565825 [IF=5.62] Lin, Xuzhu, et al. "Hindlimb Immobilization, but not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated with Muscle Atrophy in Rats." Journal of Bone and Mineral Research (2016).  WB ;  Rat.   PubMed:27291707 [IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212  WB ;  Mouse.   PubMed:10.1016/j.biocel.2022.106212 [IF=4.96] Manying Wanget al. 20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway. Biochem Pharmacol . 2020 Oct;180:114145.  WB ;  mouse.   PubMed:32653593 [IF=4.755] Zhang Lijiao. et al. Dysregulated myokines and signaling pathways in skeletal muscle dysfunction in a cigarette smoke–induced model of chronic obstructive pulmonary disease. FRONT PHYSIOL. 2022 Aug;0:1600  FC,WB ;  Mouse.   PubMed:36091368 [IF=2.893] Lijiao Zhang. et al. Myostatin/HIF2α-Mediated Ferroptosis is Involved in Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease. INT J CHRONIC OBSTR. 2022 Sep;17:2383-2399  WB ;  Mouse.   PubMed:36185172 [IF=2.59] Yamada A.K.. et al. Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway. Braz J Med Biol Res. 2022 Jan;55:  WB ;  Rat.   PubMed:35019034 [IF=2.09] Lu, Jun-Juan, et al. "Tumor necrosis factor-like weak inducer of apoptosis regulates quadriceps muscle atrophy and fiber-type alteration in a rat model of chronic obstructive pulmonary disease." Tobacco Induced Diseases 15.1 (2017): 43.  WB ;  Rat.   PubMed:29151827
Research Area	Cardiovascular  Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MuRF1: 251-353/353
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. [provided by RefSeq]. Function: E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. Subunit: Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with GNB2L1 and PRKCE. Interacts with CKM. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes. Localizes to the M- and Z-lines in skeletal muscle. Tissue Specificity: Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. Similarity: Contains 1 B box-type zinc finger. Contains 1 COS domain. Contains 1 RING-type zinc finger. SWISS: Q969Q1 Gene ID: 84676 Database links: Entrez Gene: 84676 Human Entrez Gene: 433766 Mouse Entrez Gene: 140939 Rat Omim: 606131 Human SwissProt: Q969Q1 Human SwissProt: Q38HM4 Mouse SwissProt: Q91Z63 Rat Unigene: 279709 Human Unigene: 331961 Mouse Unigene: 40636 Rat
Product Picture	Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MuRF1 Polyclonal Antibody, Unconjugated(SL2539R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:K562. Primary Antibody (green line): Rabbit Anti-MuRF1 antibody (SL2539R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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