Product Name	CD97
Chinese Name	CD97抗体
Alias	CD97 antigen subunit beta; Leukocyte antigen CD97; CD 97; CD97; CD97 antigen; CD97 molecule; CD97_HUMAN; Leukocyte antigen CD97; Seven span transmembrane protein; Seven transmembrane helix receptor; Seven transmembrane heterodimeric receptor associated with inflammation; TM7LN 1; TM7LN1.
Research Area	immunology  Neurobiology  The cell membrane受体  Cell adhesion molecule  G protein-coupled receptor  Cell Surface Molecule  glycoprotein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34/92kDa
Cellular localization	The cell membrane Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD97 antigen subunit beta: 741-835/835 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]. Function: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Subunit: Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) interacts with chondroitin sulfate. Subcellular Location: Cell membrane; Multi-pass membrane protein. CD97 antigen subunit alpha: Secreted, extracellular space. Tissue Specificity: Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid. Post-translational modifications: Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 5 EGF-like domains. Contains 1 GPS domain. SWISS: P48960 Gene ID: 976 Database links: Entrez Gene: 976 Human Entrez Gene: 26364 Mouse Entrez Gene: 361383 Rat Omim: 601211 Human SwissProt: P48960 Human SwissProt: Q9Z0M6 Mouse Unigene: 466039 Human Unigene: 334648 Mouse Unigene: 107655 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD97) Polyclonal Antibody, Unconjugated (SL2522R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD97 antibody(SL2522R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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