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Product Name SLAMF9 Chinese Name CD2F-10抗体 Alias SLAM family member 9; CD2 family member 10; CD2F-10; CD84 homolog 1; CD84-H1; 2310026I04Rik; AI462096; CD84-H1; MGC88194; SF2001; slamf9; SLAMF9; SLAF9_HUMAN. Research Area Cell biology immunology lymphocyte t-lymphocyte b-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 30kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SLAMF9: 51-150/289 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the signaling lymphocytic activation molecule family. May play a role in the immune response. The encoded protein is a cell surface molecule that consists of two extracellular immunoglobulin domains, a transmembrane domain and a short cytoplasmic tail that lacks the signal transduction motifs found in other family members. Alternative splicing results in multiple transcript variants. Expression is predominantly restricted in hematopoietic tissues. Expressed in heart, spleen, liver, intestine, muscle and testis. Expressed in immune cells, including monocytes, dendritic, B- and T-cells. No expression was seen in peripheral blood leukocytes. Expressed in the leukocyte cell line THP1-macrophage.
Function:
May play a role in the immune response.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Expression is predominantly restricted in hematopoietic tissues. Expressed in heart, spleen, liver, intestine, muscle and testis. Expressed in immune cells, including monocytes, dendritic, B- and T-cells. No expression was seen in peripheral blood leukocytes. Expressed in the leukocyte cell line THP1-macrophage.
Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
SWISS:
Q96A28
Gene ID:
89886
Database links:Entrez Gene: 89886 Human
Entrez Gene: 98365 Mouse
Omim: 608589 Human
SwissProt: Q96A28 Human
SwissProt: Q9D780 Mouse
Unigene: 661712 Human
Unigene: 150050 Mouse
Product Picture Sample:
A549 (human)cell Lysate at 40 ug
BV2 (mouse)cell Lysate at 40 ug
Primary: Anti- SLAMF9 (SL2486R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30kD
Observed band size: 35 kD
Blank control: (mo)splenocytes(blue)
Isotype Control Antibody: Rabbit IgG-FITC(orange) ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (rad line): Rabbit Anti-SLAMF9 antibody (SL2486R) ; Dilution: 1μg /10^6 cells;
Isotype Control Antibody (grenn line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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