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Product Name Cytochrome P450 2B6 Chinese Name 细胞色素P450 2B6抗体 Alias 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital inducible; Cytochrome P450 subfamily IIB (phenobarbital inducible); Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6; Cytochrome P450 subfamily IIB polypeptide 6; 1,4-cineole 2-exo-monooxygenase; IIB1; P450; P450 IIB1; P450IIB1. literatures Specific References (1) | SL2352R has been referenced in 1 publications.[IF=2.8] Man, Shuli, et al. "Combination therapy of cyclophosphamide and Rhizoma Paridis Saponins on anti-hepatocarcinoma mice and effects on cytochrome p450 enzyme expression." Steroids (2013). WB ; Rat.Research Area Tumour Cardiovascular Cell biology immunology Signal transduction Apoptosis transcriptional regulatory factor The new supersedes the old Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 56kDa Cellular localization cytoplasmic The cell membrane Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cytochrome P450 2B6: 401-491/491 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.
Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Specificity:
Expressed in liver, lung and heart right ventricle.
Post-translational modifications:
Phosphorylation is accompanied by a decrease in enzyme activity (By similarity).
Similarity:
Belongs to the cytochrome P450 family.
SWISS:
P20813
Gene ID:
1555
Database links:Entrez Gene: 1555 Human
Omim: 123930 Human
SwissProt: P20813 Human
Unigene: 1360 Human
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (SL2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (SL2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cytochrome P450 2B6 antibody (SL2352R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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