Product Name	EPO
Chinese Name	红细胞生成素抗体
Alias	Erythropoietin precursor; EP; EPO alpha; Epoetin; Erythropoetin; Erythropoietin; MGC138142; EPO_HUMAN.
literatures	Specific References  (8)     |     SL2343R has been referenced in 8 publications. [IF=7.338] Giraud S et al. The inhibition of eIF5A hypusination by GC7, a preconditioning protocol to prevent brain death‐induced renal injuries in a preclinical porcine kidney transplantation model. Am J Transplant. 2020 Dec;20(12):3326-3340.  WB ;  Pig.   PubMed:32400964 [IF=6.458] Yi-Fang Wu. et al. VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis. NANOMED-NANOTECHNOL. 2023 Feb;48:102639  WB ;  Rat.   PubMed:36549557 [IF=3.924] Pengfei Gao. et al. Pentraxin 3 promotes airway inflammation in experimental asthma. Resp Res. 2020 Dec;21(1):1-10  IF ;  Mouse.   PubMed:32938460 [IF=3.73] DeNiro, Michael, and Futwan A. Al-Mohanna. "Nuclear Factor Kappa-B Signaling Is Integral to Ocular Neovascularization in Ischemia-Independent Microenvironment." PLOS ONE 9.7 (2014): e101602.  IHC-P ;  Mouse.   PubMed:25050547 [IF=3.35] Li, Xu, et al. "Oxidative stress induces the decline of brain EPO expression in aging rats." Experimental Gerontology (2016).  WB ;  Rat.   PubMed:27452792 [IF=3.05] Ichii, Osamu, et al. "Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes." Histochemistry and Cell Biology (2016): 1-12.  WB ;  Rat.   PubMed:27099161 [IF=2.993] Desharnais P et al. Detection of Erythropoiesis Stimulating Agents in urine samples using a capillary Western system.(2018) Drug Test Anal  WB ;  Human.   PubMed:30365250 [IF=2.577] Macias-Velez RJ et al. Intranasal Erythropoietin Protects CA1 Hippocampal Cells, Modulated by Specific Time Pattern Molecular Changes After Ischemic Damage in Rats. J Mol Neurosci. 2019 May 3.  Dot-Blot ;  Rat.   PubMed:31054091
Research Area	Cardiovascular  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Erythropoietin: 101-193/193
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Subcellular Location: Secreted. Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. DISEASE: Microvascular complications of diabetes 2 (MVCD2) [MIM:612623]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Similarity: Belongs to the EPO/TPO family. SWISS: P01588 Gene ID: 2056 Database links: Entrez Gene: 404002 Dog Entrez Gene: 2056 Human Entrez Gene: 13856 Mouse Entrez Gene: 24335 Rat Omim: 133170 Human SwissProt: P33707 Dog SwissProt: P01588 Human SwissProt: P07321 Mouse SwissProt: P29676 Rat Unigene: 2303 Human Unigene: 349116 Mouse Unigene: 11365 Rat
Product Picture	Sample: Lane 1: Rat Liver tissue lysates Lane 2: Human HepG2 cell lysates Primary: Anti-EPO (SL2343R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 33 kDa Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EPO Polyclonal Antibody, Unconjugated(SL2343R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (SL2343R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (SL2343R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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