Product Name	PPAR alpha
Chinese Name	α型-过氧化酶活化增生受体抗体
Alias	hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPARA; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA_HUMAN; PPARalpha.  PPAR α; PPAR-α; PPARα;
literatures	Specific References  (17)     |     SL23398R has been referenced in 17 publications. [IF=9.988] Hao Ni. et al. Long term toxicities following developmental exposure to perfluorooctanoic acid: Roles of peroxisome proliferation activated receptor alpha. ENVIRON POLLUT. 2023 Jan;317:120722  WB ;  Chicken.   PubMed:36436667 [IF=8.071] Xiaohui Xu. et al. Hexafluoropropylene oxide dimer acid (HFPO-DA) induced developmental cardiotoxicity and hepatotoxicity in hatchling chickens: Roles of peroxisome proliferator activated receptor alpha. Environ Pollut. 2021 Dec;290:118112  WB ;  chicken.   PubMed:34500398 [IF=7.129] Qixuan Dong. et al. Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved. ECOTOX ENVIRON SAFE. 2023 Mar;253:114671  WB ;  Chicken.   PubMed:36822062 [IF=6.208] Yongzhi Sun. et al. GB1a Activates SIRT6 to Regulate Lipid Metabolism in Mouse Primary Hepatocytes. INT J MOL SCI. 2023 Jan;24(11):9540  WB ;  Human.   PubMed:37298491 [IF=6.025] Yue-Qiang Huang. et al. Di-2-ethylhexyl phthalate (DEHP) induced lipid metabolism disorder in liver via activating the LXR/SREBP-1c/PPARα/γ and NF-κB signaling pathway. FOOD CHEM TOXICOL. 2022 Jul;165:113119  WB ;  Bird.   PubMed:35537648 [IF=5.742] Pan Cao. et al. Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α. WORLD J GASTROENTERO. 2022 May 7; 28(17): 1798–1813  WB ;  Mouse,Human.   PubMed:35633910 [IF=4.96] L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemia–reperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;180:114194  WB ;  Rat.   PubMed:32800851 [IF=4.784] Pan Y et al. Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Int J Biol Macromol. 2019 Nov 8. pii: S0141-8130(19)37563-4.  WB ;  Rat.   PubMed:31712147 [IF=4.533] Huiying Gu. et al. Soluble Klotho Improves Hepatic Glucose and Lipid Homeostasis in Type 2 Diabetes. Mol Ther-Meth Clin D. 2020 Sep;18:811  WB ;  Mouse.   PubMed:32953932 [IF=4.432] Pan Cao. et al. Pinocembrin ameliorates acute liver failure via activating the Sirt1/PPARα pathway in vitro and in vivo. Eur J Pharmacol. 2022 Jan;915:174610  WB ;  Mouse.   PubMed:34951978 [IF=4.398] Xiaoyu Qu. et al. Integration of metabolomics and proteomics analysis to explore the mechanism of neurotoxicity induced by receipt of isoniazid and rifampicin in mice. NEUROTOXICOLOGY. 2023 Jan;94:24  IHC ;  Mouse.   PubMed:36347327 [IF=4.358] Jin X et al. Fine particles cause the abnormality of cardiac ATP levels via PPARɑ-mediated utilization of fattyacid and glucose using in vivo and in vitro models.Environ Pollut. 2019 Jun;249:286-294.  WB/IF ;  Rat.   PubMed:30897468 [IF=3.234] Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29.  WB ;  Mouse.   PubMed:30974116 [IF=3.188] Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.  IHF ;  Rat.   PubMed:30890453 [IF=2.629] Zang Zhen-Zhong. et al. Uncovering the Protective Mechanism of the Volatile Oil of Acorus tatarinowii against Acute Myocardial Ischemia Injury Using Network Pharmacology and Experimental Validation. Evid-Based Compl Alt. 2021;2021:6630795  WB ;  Mouse.   PubMed:34239586 [IF=2.534] Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153  WB ;  Sheep.   PubMed:34481206 [IF=1.851] Liu Q et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2. Molecular Medicine Reports.2019,19: 5087-5096 .  WB ;  Rat&Mouse.   PubMed:doi:10.3892/mmr.2019.10189.
Research Area	Tumour  immunology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	51kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PPAR alpha: 181-280/468
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. Function: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Subunit: Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity. Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2. Subcellular Location: Nucleus. Tissue Specificity: Skeletal muscle, liver, heart and kidney. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: Q07869 Gene ID: 5465 Database links: Entrez Gene: 5465 Human Entrez Gene: 19013 Mouse Entrez Gene: 25747 Rat Omim: 170998 Human SwissProt: Q07869 Human SwissProt: Q6I9S0 Human SwissProt: P23204 Mouse SwissProt: Q542P9 Mouse SwissProt: P37230 Rat Unigene: 103110 Human Unigene: 710044 Human Unigene: 212789 Mouse Unigene: 9753 Rat
Product Picture	Sample: Heart(Mouse) Lysate at 40 ug Primary: Anti- PPAR alpha (SL7114R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PPAR alpha (SL23398R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PPAR gamma 2 Polyclonal Antibody, Unconjugated(SL7114R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPAR alpha antibody (SL23398R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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