Product Name	Semaphorin 3F
Chinese Name	臂板蛋白3F抗体
Alias	Semaphorin-3F; Semaphorin3F; Sema III/F; Sema IV; sema3F; SemaIII/F; SemaIV; Semaphorin IV; SemaphorinIV; SEM3F_HUMAN.
Research Area	Developmental biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	88kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Sema3F: 201-300/785
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family. Function: May play a role in cell motility and cell adhesion. Subunit: Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Sema domain. Subcellular Location: Secreted. Tissue Specificity: Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver. SWISS: Q13275 Gene ID: 6405 Database links: Entrez Gene: 6405 Human Entrez Gene: 20350 Mouse Entrez Gene: 315996 Rat Omim: 601124 Human SwissProt: Q13275 Human SwissProt: O88632 Mouse Unigene: 32981 Human Unigene: 12903 Mouse Unigene: 205616 Rat
Product Picture	Sample: Lung (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Semaphorin 3F (SL23306R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Semaphorin 3F (SL23306R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Cerebrum(Mouse) Cell Lysate at 40 ug Primary: Anti-Semaphorin 3F (SL23306R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Hypophysis (Mouse) Lysate at 40 ug Primary: Anti- Semaphorin 3F (SL23306R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Lane 1: Mouse Brain Lysates Lane 2: Rat Brain Lysates Primary: Anti-Semaphorin 3F (SL23306R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88kDa Observed band size: 88 kDa

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Totalgoods,subtotals:￥Checkout