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Product Name IGF2R/M6PR Chinese Name 胰岛素样生长因子2受体抗体 Alias 300 kDa mannose 6-phosphate receptor; Cation independent mannose 6 phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CD222 antigen; CI Man 6 P receptor; CI Man-6-P receptor; CI MPR; CI-MPR; CIMPR; IGF 2 receptor; IGF 2R; IGF II receptor; IGF-II receptor; IGF2 receptor; IGF2R; Insulin like growth factor 2 receptor; Insulin like growth factor II receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300; MPR300; MPRI; MPRI_HUMAN. Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Cow, ) Applications ELISA=1:5000-10000 Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 300kDa Cellular localization cytoplasmic The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IGF2R/M6PR: 701-800/2491 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Function:
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Subunit:
Binds HA-I and HA-II plasma membrane adapters (By similarity). Interacts with DPP4; the interaction is direct. Binds GGA1, GGA2 and GGA3.
Subcellular Location:
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
Similarity:
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain.
SWISS:
P11717
Gene ID:
3482
Database links:
Entrez Gene: 3482 Human
Entrez Gene: 16004 Mouse
Omim: 147280 Human
SwissProt: P11717 Human
SwissProt: Q07113 Mouse
Unigene: 487062 Human
Unigene: 26553 Mouse
Product Picture Blank control (Black line): Molt4(Black).
Primary Antibody (green line): Rabbit Anti-IGF2R/M6PR antibody (SL23240R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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