TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Dystrobrevin alpha Chinese Name 肌营养蛋白α抗体 Alias DRP3; DTN; DTNA; Dystrophin related protein 3; FLJ96209; LVNC1; OTTHUMP00000163154; OTTHUMP00000163155; D18S892E; DTN-A; DTNA_HUMAN. Research Area Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Dog, Horse, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 81kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse Dystrobrevin alpha: 301-400/743 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Dystrobrevin alpha belongs to the dystrobrevin subfamily of the dystrophin family. It is a component of the dystrophin associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha and beta dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin alpha may be involved in the formation and stability of synapses as well as being involved in the clustering of nicotinic acetylcholine receptors. Mutations in Dystrobrevin alpha are associated with left ventricular noncompaction with congenital heart defects.
Function:
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration .
Subunit:
Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5. Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin.
Subcellular Location:
Cytoplasm. Cell projection, growth cone. Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.
Tissue Specificity:
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Post-translational modifications:
Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.
Similarity:
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
SWISS:
Q9D2N4
Gene ID:
13527
Database links:Entrez Gene: 1837 Human
Entrez Gene: 13527 Mouse
Omim: 601239 Human
SwissProt: Q9Y4J8 Human
SwissProt: Q9D2N4 Mouse
Unigene: 643454 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dystrobrevin alpha) Polyclonal Antibody, Unconjugated (SL23134R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Dystrobrevin alpha) polyclonal Antibody, Unconjugated (SL23134R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Scan Wechat Qrcode