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Product Name CAPN1 Chinese Name 钙蛋白酶1抗体 Alias Calcium activated neutral proteinase 1; Calpain1; Calpain-1; Calpain 1; Calpain 1 catalytic subunit; Calpain large polypeptide L1; Calpain mu type; CANP 1; CANP; CANP1; CANPL1; CAPN 1; Cell proliferation inducing gene 30 protein; Cell proliferation inducing protein 30; Micromolar calpain; muCANP; muCL; Ca2 activated neutral protease; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 (mu/I) large subunit; Calpain 1 catalytic subunit; Calpain 1 large subunit; Calpain Large Polypeptide L1; Calpain Large Polypeptide L1; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain1; CAN1_HUMAN. Research Area Cell biology Signal transduction Apoptosis Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 78kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse Calpain 1: 521-620/713 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Function:
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Subunit:
Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Subcellular Location:
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
Similarity:
Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.
SWISS:
O35350
Gene ID:
12333
Database links:Entrez Gene: 12333 Mouse
Entrez Gene: 823 Human
Omim: 114220 Human
SwissProt: P07384 Human
SwissProt: O35350 Mouse
Unigene: 502842 Human
Unigene: 6221 Mouse
Unigene: 6037 Rat
钙蛋白酶(calpain)是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括Cytoskeleton和The cell membrane附着的构型重建、不同的Signal transduction途径和Apoptosis等.Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAN1) Polyclonal Antibody, Unconjugated (SL23048R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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