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Product Name Nucleobindin 2 Chinese Name 新的饱食分子蛋白抗体 Alias DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NEFA; Nucb2; NUCB2_HUMAN; Nucleobindin-2; Nucleobinding 2. Research Area Cardiovascular Cell biology immunology Neurobiology Growth factors and hormones Diabetes Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 47kDa Cellular localization The nucleus cytoplasmic The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Nucleobindin 2: 161-250/420 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011].
Function:
Calcium-binding protein. May have a role in calcium homeostasis.
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.
Subcellular Location:
Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted. Endoplasmic reticulum. Nucleus envelope. Note=Golgi retention is mediated by its N-terminal region.
Nesfatin-1: Secreted.
Tissue Specificity:
Predominantly expressed in spleen, testis and normal stomach.
Similarity:
Belongs to the nucleobindin family.
Contains 2 EF-hand domains.
SWISS:
P80303
Gene ID:
4925
Database links:Entrez Gene: 4925 Human
Entrez Gene: 53322 Mouse
Omim: 608020 Human
SwissProt: P80303 Human
SwissProt: P81117 Mouse
Unigene: 654599 Human
Unigene: 9901 Mouse
Unigene: 41602 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nucleobindin 2) Polyclonal Antibody, Unconjugated (SL23043R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUCB2) Polyclonal Antibody, Unconjugated (SL23043R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUCB2) Polyclonal Antibody, Unconjugated (SL23043R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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