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Product Name Integrin alpha E Chinese Name 整合素alpha E2(CD103)抗体 Alias Itgae; A530055J10; alpha-E1; alpha-M290; aM290; CD103; MGC183601; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; ITAE_HUMAN; Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; Integrin alpha-E heavy chain; integrin alpha-E precursor. Research Area Cell biology immunology Signal transduction Stem cells Cell adhesion molecule Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 110/128kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Integrin alpha-E heavy chain: 1101-1179/1179 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]
Function:
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Subunit:
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Tissue Specificity:
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
SWISS:
P38570
Gene ID:
3682
Database links:Entrez Gene: 3682 Human
Omim: 604682 Human
SwissProt: P38570 Human
Unigene: 513867 Human
Product Picture Sample:
U937(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha E2 (SL2217R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kD
Observed band size: 110 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-Integrin alpha E2 (SL2217R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kD
Observed band size: 128 kD
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD103 Antibody(SL2217R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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