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Product Name IL-1RA Chinese Name 白介素-1受体拮抗剂抗体 Alias Interleukin-1 receptor antagonist; ICIL 1RA; IL1 inhibitor; ICIL1RA; IL1F3; IL1RN; IRAP; IL1RA_HUMAN; IL1RA_MOUSE. Research Area immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 17kDa Cellular localization cytoplasmic Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse IL-1RA: 26-120/178 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Function:
Inhibits the activity of interleukin-1 by binding to eceptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Subcellular Location:
Isoform 1: Secreted.
Isoform 2, 3, 4: Cytoplasm.
Tissue Specificity:
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
DISEASE:
Microvascular complications of diabetes 4 (MVCD4) [MIM:612628]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]: A rare autoinflammatory disease of skin and bone resulting in sterile multifocal osteomyelitis, periostitis, and pustulosis from birth. The term autoinflammatory disease describes a group of disorders characterized by attacks of seemingly unprovoked inflammation without significant levels of autoantibodies and autoreactive T-cells. Note=The disease is caused by mutations affecting the gene represented in this entry.
SWISS:
P25085
Gene ID:
16181
Database links:Entrez Gene: 3557 Human
Entrez Gene: 16181 Mouse
Omim: 147679 Human
SwissProt: P18510 Human
SwissProt: P25085 Mouse
Unigene: 81134 Human
Unigene: 882 Mouse
Unigene: 162640 Rat
IL-1受体拮抗剂(IL-1ra)是IL-1强有力的内生拮抗剂,通过与白细胞介素-1的Ⅰ型和Ⅱ型受体结合,特异性地阻断白细胞介素-1的生物学作用。白介素1受体拮抗剂(IL-1Ra)是体内天然的一种对IL-1有拮抗作用的cell factor。Product Picture Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug
Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug
Primary: Anti-IL-1RA (SL2216R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 23 kD
Sample: Spleen(Mouse) Tissue Lysate at 30 ug
Primary: Anti-IL-1RA (SL2216R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (SL2216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(SL2216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (SL2216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:A431.
Primary Antibody (green line): Rabbit Anti-IL-1RA antibody (SL2216R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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