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Product Name PAMCI/RASF9 Chinese Name RAS家族关联结构域蛋白9抗体 Alias P CIP1; P-CIP1; PAM COOH-terminal interactor protein 1; PCIP; Peptidylglycine alpha amidating monooxygenase COOH terminal interactor; peptidylglycine alpha-amidating monooxygenase COOH terminal interactor protein 1; Peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor; Ras association (RalGDS/AF 6) domain family (N terminal) member 9; Ras association domain-containing protein 9; RASF9_HUMAN; Rassf9. Research Area Cell biology Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Cow, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PAMCI/RASF9: 1-100/435 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene localizes to perinuclear endosomes. This protein associates with peptidylglycine alpha-amidating monooxygenase, and may be involved with the trafficking of this enzyme through secretory or endosomal pathways. [provided by RefSeq, Jul 2008]
Function:
May play a role in regulating vesicuar trafficking in cells.
Subcellular Location:
Endosome. Accumulates on perinuclear endosomes.
Similarity:
Contains 1 Ras-associating domain.
SWISS:
O75901
Gene ID:
9182
Database links:Entrez Gene: 9182 Human
Entrez Gene: 237504 Mouse
Omim: 610383 Human
SwissProt: O75901 Human
SwissProt: Q8K342 Mouse
Unigene: 527881 Human
Unigene: 282672 Mouse
Unigene: 30013 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAMCI/RASF9) Polyclonal Antibody, Unconjugated (SL21011R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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