Product Name	DDIT3
Chinese Name	GADD153抗体
Alias	GADD153; CHOP; Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.
literatures	Specific References  (14)     |     SL20669R has been referenced in 14 publications. [IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039  WB ;  Mouse.   PubMed:33511306 [IF=7.675] Reziyamu Wufuer. et al. Distinct Roles of Nrf1 and Nrf2 in Monitoring the Reductive Stress Response to Dithiothreitol (DTT). ANTIOXIDANTS-BASEL. 2022 Aug;11(8):1535  WB ;  Human.   PubMed:36009254 [IF=6.304] Biting Zhou. et al. Mitochondrial quality control protects photoreceptors against oxidative stress in the H 2 O 2 -induced models of retinal degeneration diseases. Cell Death Dis. 2021 Apr;12(5):1-12  WB ;  Mouse.   PubMed:33879768 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242  WB ;  Pig.   PubMed:37108405 [IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551  WB ;  Mouse.   PubMed:32996162 [IF=5.194] Li Wang. et al. New sight into interaction between endoplasmic reticulum stress and autophagy induced by vanadium in duck renal tubule epithelial cells. CHEM-BIOL INTERACT. 2022 May;:109981  WB ;  Duck.   PubMed:35588787 [IF=4.52] Zhu YP et al. Unification of Opposites between Two Antioxidant Transcription Factors Nrf1 and Nrf2 in Mediating Distinct Cellular Responses to the Endoplasmic Reticulum Stressor Tunicamycin. Antioxidants (Basel). 2019 Dec 19;9(1). pii: E4.  WB ;  Human.   PubMed:31861550 [IF=4.155] Quanwei Li. et al. Toxicological mechanism of large amount of copper supplementation: Effects on endoplasmic reticulum stress and mitochondria-mediated apoptosis by Nrf2/HO-1 pathway-induced oxidative stress in the porcine myocardium. J Inorg Biochem. 2022 May;230:111750  WB ;  Pig.   PubMed:35151098 [IF=4.014] Xueping Chen. et al. Baicalin ameliorates Mycoplasma gallisepticum-induced inflammatory injury via inhibiting STIM1-regulated ceramide accumulation in DF-1 cells. POULTRY SCIENCE. 2023 Mar;:102687  WB ;  Chicken.   PubMed:10.1016/j.psj.2023.102687 [IF=3.606] Xuejiao Zhou. et al. The novel ALK inhibitor ZX‐29 induces apoptosis through inhibiting ALK and inducing ROS‐mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02  WB ;  Human.   PubMed:33140567 [IF=3.159] Tao Tu. et al. Dietary ω-3 fatty acids reduced atrial fibrillation vulnerability via attenuating myocardial endoplasmic reticulum stress and inflammation in a canine model of atrial fibrillation. J Cardiol. 2021 Oct;:  WB ;  Dog.   PubMed:34702603 [IF=3.046] Yong-yi Dai. et al. Effects of exogenous vitamin D on endoplasmic reticulum stress-related factors in the vitamin D deficiency rats with cerebral ischemia. Neurosci Lett. 2021 Jul;:136115  WB ;  Rat.   PubMed:34274437 [IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15.  ICF&WB ;  Mouse.   PubMed:30114659 [IF=2.686] Hu, Yikai. et al. ER stress–related protein, CHOP, may serve as a biomarker of mechanical asphyxia: a primary study. Int J Legal Med. 2022 Feb;:1-14  WB,IF ;  Rat,Human.   PubMed:35122137
Research Area	immunology  Signal transduction  Apoptosis  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	19kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GADD153/DDIT3: 81-150/168
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]. Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Subunit: Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4. Subcellular Location: Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation. Post-translational modifications: Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity. DISEASE: Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. Similarity: Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P35638 Gene ID: 1649 Database links: Entrez Gene: 1649 Human Entrez Gene: 13198 Mouse Entrez Gene: 29467 Rat Omim: 126337 Human SwissProt: P35638 Human SwissProt: P35639 Mouse SwissProt: Q62857 Rat Unigene: 505777 Human GADD153蛋白与Apoptosis和死亡密切相关,在某种程度上决定了细胞周期的进程。
Product Picture	Sample: TT(Human) Cell Lysate at 40 ug Primary: Anti-DDIT3 (SL20669R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD ample: K562 (Human) Cell Lysate at 30 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-DDIT3 (SL20669R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19’27 kD Observed band size: 19 kD Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDIT3) Polyclonal Antibody, Unconjugated (SL20669R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDIT3) Polyclonal Antibody, Unconjugated (SL20669R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-DDIT3 antibody (SL20669R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

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