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Product Name NKG2D Chinese Name NK细胞受体2D抗体 Alias KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD 314; CD314 antigen; D12S2489E; Killer cell lectin like receptor subfamily K member 1; Killer cell lectin-like receptor subfamily K member 1; KLR; Klrk1; NKG2 D activating NK receptor; NKG2 D type II integral membrane protein; NKG2-D-activating NK receptor; NKG2D_HUMAN; NKLLR; NKR P2; Nkrp2. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Pig, ) Applications WB=1:500-2000 Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 25kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NKG2D: 101-200/219 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]
Function:
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
Subunit:
Homodimer.
Subcellular Location:
Membrane; Single-pass type II membrane protein.
Tissue Specificity:
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
Similarity:
Contains 1 C-type lectin domain.
SWISS:
P26718
Gene ID:
22914
Database links:Entrez Gene: 22914 Human
Entrez Gene: 27007 Mouse
Omim: 611817 Human
SwissProt: P26718 Human
SwissProt: O54709 Mouse
Unigene: 387787 Human
Unigene: 8217 Mouse
Unigene: 14544 Rat
NKG2-D出现在免疫细胞上表达的受体,NKG2D属C型凝集素家族Transmembrane protein,广泛表达在NK细胞、CD8+的αβT细胞和γδT细胞表面,可以提高NK细胞对Tumour细胞的杀伤活性。Product Picture Sample:
Lung (Mouse) Lysate at 40 ug
Raw246.7(Mouse) Cell Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Bone (Rat) Lysate at 40 ug
Primary: Anti- NKG2D (SL20660R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-NKG2D(SL20660R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25kD
Observed band size: 26kD
Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells ).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL20660R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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