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Product Name GPR30 Chinese Name G protein-coupled receptor30抗体 Alias G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER. Research Area Cell biology Neurobiology Signal transduction The cell membrane受体 G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization The nucleus cytoplasmic The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GPR30: 21-120/375 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GRP30 is a member of this family and is an orphan receptor. GPR30 expression has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. In brain, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. However, unlike other known G protein-coupled receptors, GPR30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives.
Function:
Receptor for estrogen.
Subcellular Location:
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
Tissue Specificity:
Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
Q99527
Gene ID:
2852
Database links:Entrez Gene: 2852 Human
Entrez Gene: 76854 Mouse
Omim: 601805 Human
SwissProt: Q99527 Human
SwissProt: Q8BMP4 Mouse
Unigene: 20961 Human
Unigene: 389706 Mouse
Unigene: 9806 Rat
GPR30是一种膜性雌激素蛋白受体又称非基因型雌激素膜性受体GPR30。GPR30受体广泛表达于海马、下丘脑、子宫、卵巢、乳腺、骨和Cardiovascular等全身多个系统、器官和组织,在细胞内主要定位于The cell membrane、内质网、Mitochondrion和高尔基体。 GPER与雌激素结合, ERK、PI3K/AKT)、 cAMP等第二信使途径发挥快速非基因效应,在神经系统、生殖系统、运动系统和Cardiovascular系统中发挥重要的生理作用。Product Picture Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Fetal brain (Mouse) Lysate at 40 ug
Lane 3: Large intestine (Mouse) Lysate at 40 ug
Lane 4: Uterus (Mouse) Lysate at 40 ug
Lane 5: Heart (Mouse) Lysate at 40 ug
Lane 6: Large intestine (Rat) Lysate at 40 ug
Lane 7: Uterus (Rat) Lysate at 40 ug
Lane 8: Heart (Rat) Lysate at 40 ug
Primary: Anti-GPR30 (SL20642R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20642R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated (SL20642R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-PR30 antibody (SL20642R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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