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Product Name CD90/Thy-1 Chinese Name CD90抗体 Alias CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN. literatures Specific References (6) | SL20640R has been referenced in 6 publications.[IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039 IF,IHC ; Mouse.[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359 IF ; Mouse.[IF=7.242] Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 Mar;: IF ; Mouse.[IF=6.353] Chen Chong. et al. 3D printed collagen/silk fibroin scaffolds carrying the secretome of human umbilical mesenchymal stem cells ameliorated neurological dysfunction after spinal cord injury in rats. Regen Biomater. 2022 Feb;: IF ; Human.[IF=5.999] Jinsheng Li. et al. Micro/nano-topography Promotes Osteogenic Differentiation of Bone Marrow Stem Cells by Regulating Periostin Expression. COLLOID SURFACE B. 2022 Jul;:112700 FC ; Rat.[IF=2.092] Yuan H et al. MicroRNA let-7c-5p promotes osteogenic differentiation of dental pulp stem cells by inhibiting lipopolysaccharide-induced inflammation via HMGA2/PI3K/Akt signal blockade. Clinical and Experimental Pharmacology and Physiology.2018 doi:10.1111/1440-1681.13059 ICF ; Rat.Research Area Cell biology immunology Neurobiology t-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 12kDa Detection molecular weight 25-35kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CD90: 20-100/161 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity:
Abundant in lymphoid tissues.
Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
P04216
Gene ID:
7070
Database links:Entrez Gene: 7070 Human
Entrez Gene: 21838 Mouse
Omim: 188230 Human
SwissProt: P04216 Human
SwissProt: P01831 Mouse
Unigene: 644697 Human
Unigene: 3951 Mouse
Unigene: 108198 Rat
Thy1/CD90主要表达于脑和淋巴组织,包括胸腺细胞、外周血液T细胞和一些上皮内层T细胞。
Thy-1是一种糖基磷酸酯酰醇(GPI)固定的表面glycoprotein,Thy-1在T细胞激活、神经系统发育及其发挥功能、细胞程序性死亡等方面发挥作用。Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (SL20640R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 12 kD
Blank control:Mouse brain.
Primary Antibody (green line): Rabbit Anti-CD90 antibody (SL20640R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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