Product Name	CD33
Chinese Name	CD33抗体
Alias	CD33_HUMAN; CD33 antigen; CD33 molecule; FLJ00391; gp67; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3.
literatures	Specific References  (1)     |     SL2042R has been referenced in 1 publications. [IF=6.04] An, J. J., et al. "The ROS/JNK/ATF2 pathway mediates selenite-induced leukemia NB4 cell cycle arrest and apoptosis in vitro and in vivo." Cell Death & Disease 4.12 (2013): e973.  IHC-P ;  Human.   PubMed:24357804
Research Area	immunology  Neurobiology  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40/25kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD33: 261-364/364 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome. [provided by Alliance of Genome Resources, Apr 2022] Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Subunit: Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Monocytic/myeloid lineage cells. Post-translational modifications: Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P20138 Gene ID: 945 Database links: Entrez Gene: 945 Human Entrez Gene: 12489 Mouse Entrez Gene: 690492 Rat Omim: 159590 Human SwissProt: P20138 Human SwissProt: Q63994 Mouse Unigene: 83731 Human Unigene: 140157 Mouse CD33(Siglec-3)也是急性lymphocyte白血病抗原,细胞表面跨膜glycoprotein.CD33表达于早期Blymphocyte,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的Tumour细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.
Product Picture	Sample: Jurkat(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at30 ug Primary: Anti-CD33 (SL2042R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/25 kD Observed band size: 67 kD Blank control: U937 (blue). Primary Antibody:Rabbit Anti- CD33 antibody(SL2042R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL2042R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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